Journal: Cell biochemistry and biophysics

Article Title: Epithelial-mesenchymal Transition (EMT) and the Effect of Atorvastatin on it in ARPE-19 cells.

doi: 10.1007/s12013-024-01305-w

Figure Lengend Snippet: Fig. 8 The atorvastatin (ATV) effect on the levels of α-SMA protein in ARPE-19 cells without or with EMT induction. DMSO served as dilu- tion control to atorvastatin. The quantitation of the western blot analysis (A, n = 4) and a representative membrane image (B) of α-SMA at day 5. Relative protein expression was normalized to the internal protein control GAPDH. Untreated control was set to 1 (A), and other groups were compared to that. The bars indicate means ± SEM. Mann–Whitney U‐ test, *P < 0.05; ns, not significant (Mann–Whitney U‐test)

Article Snippet: Washing three times (á 300 μl) with 0.1% BSA in DPBS preceded the addition of mouse monoclonal αSMA primary antibody (1:125; Cat. MAB1420, R&D Systems) or sheep polyclonal fibronectin antibody (1:500; Cat. AF1918, Bio-Techne) in 1% BSA and 0.1% Triton-X in DPBS overnight at +4 °C or for 90 min at room temperature, respectively.

Techniques: Control, Quantitation Assay, Western Blot, Membrane, Expressing, MANN-WHITNEY